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KMID : 1123920050190041046
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Korean Journal of Oriental Physiology and Pathology
2005 Volume.19 No. 4 p.1046 ~ p.1049
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Discrimination and Genetic Diversity of Acanthopanax senticosus Using RAPD Markers
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Huh Man-Kyu
Choi Yung-Hyun
Choi Byung-Tae
Kim Gyeong-Cheol
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Abstract
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Acanthopanx senticosus (Araliaceae) is a long-lived woody species primarily distributed throughout East Asia. This species is regarded as medically and ecologically important woody plants in Korea. To identify the variation of the RAPD patterns between domestic and foreign A. senticosus species, 22 random primers were applied to Korean A. senticosus and A. senticosus for. inermis, Chinese and Russian A. senticosus. Six primers of them could be used to discriminate the origins and 58 polymorphisms among 92 scored DNA fragments. Six bands are specific for Korean A. senticosus and A. senticosus for. inermis. Especially, three primers, OPD04, OPD11 and OPE10, were useful to differentiate between domestic and foreign Acanthopanax species. RAPD analysis was a useful method to discriminate among A. senticosus populations or accessions and Korean accessions are distinct genetically.
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KEYWORD
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Acanthopanax species, discrimination, RAPD
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